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Reference Number:
3676CA_CellBiology_TK
Job Description:
Experience in the use of FACS-based assays to measure protein expression on the surface of bacteria and/ or mammalian cells is essential. The use of biochemical and/ or functional assays relevant to immunology and bacterial infectivity (e.g. complement fixation, functional killing assays), protein expression analysis using Western Blot and/ or ELISA, in vitro culture of BSL2 bacteria, maintenance and differentiation of mammalian cell lines, molecular cloning would also be advantageous.
Position Purpose
? Vaccine Research seeks to discover novel vaccines (prophylactic and therapeutic) for the prevention and treatment of significant human diseases (infectious diseases, cardiovascular, oncology, CNS, asthma and inflammation) using a variety of vaccine technology platforms combined with immunomodulatory agents.
? As a cell biologist with experience working with infectious organisms, the individual will use their technical skills and knowledge to support a range of assays and cell provision needs in the Vaccine Research West group in La Jolla.
? Experience in one or more of the following areas is essential ? use of FACS-based assays to measure protein expression on the surface of bacteria and/ or mammalian cells; use of biochemical and/ or functional assays relevant to immunology and bacterial infectivity (e.g. complement fixation, functional killing assays), protein expression analysis using Western Blot and/ or ELISA, in vitro culture of BSL2 bacteria, maintenance and differentiation of mammalian cell lines, molecular cloning.
? This is a primarily a laboratory based role. The individual will be required to work with moderate/ limited supervision and will be accountable for the delivery and critical appraisal of their results and presenting them for review at meetings.
Primary Duties:
- Laboratory and related work (80-90% of time) under daily to weekly supervision.
- Work with supervisor to define aim of experiments and a plan to achieve that aim in a given time frame, will then execute work following the plan (independently, if familiar with area of work, with guidance if unfamiliar).
- Experimental design ? will fine tune experiments following discussion with supervisor and literature review, with increasing emphasis on independent design based on experience.
- Will carry out on-going analysis of data generated and will identify problems. Will implement necessary changes following discussion with peers/ supervisor.
- Will analyze and interpret experimental data, results and methodology used with appropriate guidance.
Documentation:
- Contribute to writing of e.g. SOPs, study reports and protocols.
- Write up laboratory notebooks regularly in compliance with GSP or GLP if relevant.
- Preparation of presentation materials.
Safe working and regulatory compliance:
- Work safely themselves.
- Completion of risk assessments, compliance with PGRD procedures.
- Will recognize risks (own and others), within own area of expertise. Intervene or take action, where appropriate.
Training & Education (Minimum Requirements):
B.Sc. or M.Sc. in life sciences, preferably in bacteriology/ microbiology/ immunology
- Ideally this would equate to at least 3 years working in a laboratory in either an academic or industrial setting
- Flow Cytommetry is a must have (FACS based assays)
- Previous experience in Vaccines and/or infectious diseases (bacterial) is highly desired
Experience Level:
Unspecified
Requirements:
Experience in the use of FACS-based assays to measure protein expression on the surface of bacteria and/ or mammalian cells is essential.
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